ispD [2019-07-19 10:16:59]

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, third step in the MEP pathway of isoprenoid biosynthesis

Locus: BSU_00900

Isoelectric point: 5.58

Molecular weight: 25.70 kDa

Protein length: 232 aa

Gene length: 699 bp

Function: MEP pathway of isoprenoid biosynthesis

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Essential: yes

E.C.: 2.7.7.60

Synonyms: yacM

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of isoprenoids
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Essential genes

Gene

Coordinates: 109,789 → 110,487

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)

Protein family

• cytidylyltransferase family (with TagD and YfnH, according to UniProt)
• IspD/TarI cytidylyltransferase family (single member, according to UniProt)

Structure

• 5DDT PubMed
• 5DDV PubMed

Expression and Regulation

Operons

• Genes: yacL-ispD-ispF
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon
  • SigM: sigma factor, PubMed, in SigM regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-B881 (yacM::erm), available at the NBRP B. subtilis, Japan

References

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